Rabbit Anti-Iroquois homeobox protein 3 antibody

Home > Product > Antibody > Rabbit Anti-Iroquois homeobox protein 3 antibody

IRXB1; Homeodomain protein IRXB1; Iroquois homeobox protein 3; Iroquois-class homeodomain protein IRX-3; IRX 1; Irx3; IRX3_HUMAN; AI894186.

Cat:

SL7867R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human Iroquois homeobox protein 3:401-501/501

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- Iroquois homeobox protein 3 (SL7867R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 53 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Iroquois homeobox protein 3 Polyclonal Antibody, Unconjugated(SL7867R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.

Function:
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells (By similarity).

Subunit:
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
P78415

Gene ID:
79191

Database links:
UniProtKB/Swiss-Prot: P78415.3

Entrez Gene: 79191 Human

Entrez Gene: 16373 Mouse

Omim: 612985 Human

SwissProt: P78415 Human

SwissProt: P81067 Mouse

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Iroquois homeobox protein 3 Polyclonal Antibody, Unconjugated(SL7867R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall