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Home > Product > Antibody > Rabbit Anti-NIFK antibody
hNIFK; MKI67 FHA Domain Interacting Nucleolar Phosphoprotein; MKI67IP; NOPP34; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI 67; pKi67; MK67I_HUMAN.
Cat:
SL7937R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human NIFK:53-150/293
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NIFK) Polyclonal Antibody, Unconjugated (SL7937R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NIFK) Polyclonal Antibody, Unconjugated (SL7937R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009].

Subunit:
Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.

Subcellular Location:
Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67.

Post-translational modifications:
Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
Q8TB66

Gene ID:
84365

Database links:

Entrez Gene: 84365 Human

Entrez Gene: 67949 Mouse

Omim: 611970 Human

SwissProt: Q8TB66 Human

SwissProt: Q9BYG3 Human

SwissProt: Q91VE6 Mouse

Unigene: 367842 Human

Unigene: 280311 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NIFK) Polyclonal Antibody, Unconjugated (SL7937R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NIFK) Polyclonal Antibody, Unconjugated (SL7937R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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