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Home > Product > Antibody > Rabbit Anti-AMN1 antibody
AMN1; AMN1_HUMAN; Antagonist of mitotic exit network 1 homolog; Protein AMN1 homolog.
Cat:
SL7960R
Species Reactivity：
Rat,(predicted: Human,Mouse,Chicken,Dog,Cow,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human AMN1:1-100/258
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AMN1 Polyclonal Antibody, Unconjugated(SL7960R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AMN1 Polyclonal Antibody, Unconjugated(SL7960R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


AMN1 is a 258 amino acid protein belonging to the AMN1 family. Encoded by a gene that maps to human chromosome 12p11.21, AMN1 contains twelve degenerate leucine-rich repeat (LRR) motifs and modulates Ste12 binding to promoters of multiple genes. As a daughter cell-specific protein, AMN1 inhibits ELL function to deactivate the mitotic exit state and reset the cell cycle in G1. AMN1 does not halt mitotic exit, but turns it off afterward. Induced by ELL after exit activation, AMN1 disrupts TEM1-Cdc15 interaction by competing with Cdc15 for TEM1 binding and by facilitating inactivation of Cdc14. Defects in AMN1 can result in disruption of both the spindle assembly and nuclear orientation checkpoints. AMN1 may also mediate gene activity and phenotypic diversity.

Similarity:
Belongs to the AMN1 family.

SWISS:
Q8IY45

Gene ID:
196394

Database links:

Entrez Gene: 524465 Cow

Entrez Gene: 196394 Human

Entrez Gene: 302032 Rat

SwissProt: Q32L08 Cow

SwissProt: Q8IY45 Human

SwissProt: Q5U201 Rat

Unigene: 585084 Human

Unigene: 591146 Human

Unigene: 104358 Rat



Picture

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AMN1 Polyclonal Antibody, Unconjugated(SL7960R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AMN1 Polyclonal Antibody, Unconjugated(SL7960R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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