Home > Product > Antibody > Rabbit Anti-CCDC83 antibody
Coiled coil domain containing 83; Coiled coil domain containing protein 83; HSD9; QtsA 10152; QtsA 19320; CCD83_HUMAN.
Cat:
SL8139R
Species Reactivity:
Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human CCDC83:101-200/413
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample:293T Cell(Human) Lysate at 40 ugPrimary: Anti-CCDC83(SL8139R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 46kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC83) Polyclonal Antibody, Unconjugated (SL8139R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


The coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion, and drug extrusion and delivery. CCDC83 (coiled-coil domain-containing protein 83), also known as HSD9, is 413 amino acid protein that exists as three alternatively spliced isoforms. The gene encoding CCDC83 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

SWISS:
Q8IWF9

Gene ID:
44047

Database links:

Entrez Gene: 44047 Human

Entrez Gene: 617342 Cow

SwissProt: Q2TA00 Cow

SwissProt: Q8IWF9 Human

Unigene: 567774 Human



Picture

Sample:293T Cell(Human) Lysate at 40 ug
Primary: Anti-CCDC83(SL8139R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 46kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC83) Polyclonal Antibody, Unconjugated (SL8139R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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