Rabbit Anti-DPH2 antibody | Sunlong Medical

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Diphthamide biosynthesis like protein 2; Diphthamide biosynthesis protein 2; Diphthamide biosynthesis protein 2 homolog like 2; Diphthamide biosynthesis protein 2 homolog-like 2; Diptheria toxin resistance protein required for diphthamide biosynthesis lik

Cat:

SL8282R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human DPH2:401-489/489

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti-DPH2 (SL8282R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 60 kDSample:Hela Cell (Human) Lysate at 30 ugMCF-7Cell (Human) Lysate at 30 ugPrimary: Anti-DPH2 (SL8282R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52kDObserved band size: 52kD

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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DPH2 (diphthamide biosynthesis protein 2), also known as DPH2L2, is a 489 amino acid protein that shows strong expression in skeletal muscle; moderate expression in heart, small intestine, liver, pancreas, testis and colon; and lesser expression in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. DPH2 interacts with DPH1 and, functioning together as a dimer or multimer, DPH1 and DPH2 may participate in diphthamide biosynthesis. Diphthamide is a posttranslationally modified histidine residue which occurs in EF-2 (elongation factor 2) and targets diphtheria toxin ADP-ribosylation. The loss of DPH2 in Saccharomyces cerevisiae is believed to suppress zymocicity. Two transcript variants encoding different isoforms have been found for this gene.
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.
Tissue specificity:
Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.

Function:
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (By similarity).

Subunit:
Interacts with DPH1 (By similarity).

Tissue Specificity:
Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.

Similarity:
Belongs to the DPH1/DPH2 family. DPH2 subfamily.

SWISS:
Q9BQC3

Gene ID:
362

Database links:
UniProtKB/Swiss-Prot: Q9BQC3.1

Picture

Sample:
Hela Cell (Human) Lysate at 30 ug
MCF-7Cell (Human) Lysate at 30 ug
Primary: Anti-DPH2 (SL8282R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD

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