Rabbit Anti-UBE2D4 antibody

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HBUCE 1; HBUCE 1; HBUCE1; UB2D4_HUMAN; UBE2D 4; UBE2D4; Ubiquitin carrier protein D4; Ubiquitin conjugating enzyme 1 isoform; Ubiquitin-conjugating enzyme E2 D4; Ubiquitin-protein ligase D4.

Cat:

SL8354R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human UBE2D4:31-130/147

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549 Cell (Human) Lysate at 40 ugPrimary: Anti-UBE2D4 (SL8354R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 17 kDObserved band size: 18 kDParaformaldehyde-fixed, paraffin embedded Rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UBE2D4 Polyclonal Antibody, Unconjugated (SL8354R) at 1:600 overnight at 4°C, DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin SLCterminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation.

Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.

Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

SWISS:
Q9Y2X8

Gene ID:
51619

Database links:
UniProtKB/Swiss-Prot: Q9Y2X8.1

Picture

Paraformaldehyde-fixed, paraffin embedded Rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UBE2D4 Polyclonal Antibody, Unconjugated (SL8354R) at 1:600 overnight at 4°C, DAB staining.

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