Tripartite motif-containing protein 41 (TRIM41), also known as RINCK, is a 630 amino acid member of the TRIM family, also known as the RING-B-box coiled-coil (RBCC) family. Members of the RBCC family have an N-terminal RING finger, followed by one or two zinc-binding domains (B-box domains), a leucine coiled-coil region and a variable SLCterminal domain. Localized to both the nucleus and cytoplasm, TRIM41 associates with protein kinase C (PKC) through the C1A domain of PKC. Studies have shown that overexpression of TRIM41 reduces the levels of PKC in cells, whereas knockdown of TRIM41 leads to increased levels of PKC. Thus, it is hypothesized that TRIM41 plays a role in regulating PCK levels in cells, specifically through the ubiquitination of PKC. Four isoforms of TRIM41 exist as a result of alternative splicing events.
Function:
Functions as an E3 ligase that catalyzes the ubiquitin-mediated degradation of protein kinase C.
Subunit:
Interacts with PRKCA.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Expressed in multiple tissues with the highest levels in heart and skeletal muscle.
Post-translational modifications:
Auto-ubiquitinated.
Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
SWISS:
Q8WV44
Gene ID:
90933
Database links:
Entrez Gene: 90933 Human
Entrez Gene: 211007 Mouse
Entrez Gene: 303088 Rat
Omim: 610530 Human
SwissProt: Q8WV44 Human
SwissProt: Q5NCC3 Mouse
Unigene: 441488 Human
Unigene: 794 Mouse
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM41) Polyclonal Antibody, Unconjugated (SL9151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TRIM41 Polyclonal Antibody, Unconjugated(SL9151R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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