Rabbit Anti-RNF13 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-RNF13 antibody

E3 ubiquitin-protein ligase RNF13; FLJ93817; MGC13689; RING finger protein 13; Ring zinc finger protein; RNF 13; Rnf13; RNF13_HUMAN; RZF.

Cat:

SL9158R

Species Reactivity：

Mouse,Rat,(predicted: Human,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF13:201-300/381

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(SL9158R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingissue/cell: bone of mouse embryo;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(SL9158R) 1:200, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF13 (ring finger protein 13), also known as RZF, FLJ93817 or MGC13689, is a novel 381 amino acid E3 ubiquitin ligase that localizes to the nucleus. RNF13 contains one RING-type zinc finger and the SLCterminal portion of RNF13 has the ability to mediate ubiquitination. Recent studies suggest that RNF13 may be involved in the development of pancreatic cancer via ubiquitin-mediated modification of proteins. The gene encoding RNF13 maps to human chromsome 3q25.1, and a pseudogene (which is also located on chromosome 3), exists for this gene.

Function:
E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.

Subcellular Location:
Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.

Tissue Specificity:
Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).

Post-translational modifications:
Auto-ubiquitinated.

Similarity:
Contains 1 PA (protease associated) domain.
Contains 1 RING-type zinc finger.

SWISS:
O43567

Gene ID:
11342

Database links:

Entrez Gene: 11342 Human

Omim: 609247 Human

SwissProt: O43567 Human

Unigene: 12333 Human

Picture

issue/cell: bone of mouse embryo;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-RNF13 Polyclonal Antibody, Unconjugated(SL9158R) 1:200, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Product Feedback Wall