The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin SLCterminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Function:
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Subunit:
Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.
Subcellular Location:
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
Tissue Specificity:
Expressed in all tissues examined, with strongest expression in pancreas.
Similarity:
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger.
SWISS:
Q9Y6I4
Gene ID:
9960
Database links:
Entrez Gene: 9960 Human
Entrez Gene: 235441 Mouse
Entrez Gene: 363084 Rat
GenBank: NM_006537 Human
GenBank: NM_144937 Mouse
Omim: 604728 Human
SwissProt: Q9Y6I4 Human
SwissProt: Q91W36 Mouse
SwissProt: A1L1I5 Rat
Unigene: 458499 Human
Unigene: 38976 Mouse
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Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- USP3 (SL966R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Sample:
A549(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
Primary: Anti- USP3 (SL966R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
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