Rabbit Anti-RNF70 antibody | Sunlong Medical

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E3 ubiquitin-protein ligase Praja 1; E3 ubiquitin-protein ligase Praja-1; PJA1; PJA1_HUMAN; PRAJA 1; Praja ring finger 1; Praja1; Ring Finger Protein 70.

Cat:

SL9223R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF70:541-640/643

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugPrimary: Anti-RNF70 (SL9223R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 71 kDObserved band size: 71 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF70) Polyclonal Antibody, Unconjugated (SL9223R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.

Function:
Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.

Subunit:
Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2.

Tissue Specificity:
Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.

Post-translational modifications:
Substrate for E2-dependent ubiquitination.

Similarity:
Contains 1 RING-type zinc finger.

SWISS:
Q8NG27

Gene ID:
64219

Database links:

Entrez Gene: 64219 Human

Omim: 300420 Human

SwissProt: Q8NG27 Human

Unigene: 522679 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF70) Polyclonal Antibody, Unconjugated (SL9223R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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