Rabbit Anti-RNF156 antibody

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E3 ubiquitin-protein ligase MGRN1; KIAA0544; Mahogunin ring finger 1; Mahogunin RING finger protein 1; MGRN 1; MGRN1; MGRN1_HUMAN; Probable E3 ubiquitin protein ligase MGRN1; RING finger protein 156; RNF 156.

Cat:

SL9229R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF156:201-300/552

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (SL9229R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB stainingParaformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (SL9229R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.

Function:
E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.

Subunit:
Interacts with MC1R and MC4R, but not with TBXA2R. Interacts with TSG101. Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement (By similarity).

Subcellular Location:
Cytoplasm. Cell membrane.

Post-translational modifications:
Autoubiquitinated in vitro (By similarity).

Similarity:
Contains 1 RING-type zinc finger.

SWISS:
O60291

Gene ID:
23295

Database links:

Entrez Gene: 23295 Human

Entrez Gene: 17237 Mouse

Entrez Gene: 302938 Rat

Omim: 607559 Human

SwissProt: O60291 Human

SwissProt: Q9D074 Mouse

SwissProt: Q5XIQ4 Rat

Unigene: 526494 Human

Unigene: 291326 Mouse

Unigene: 15530 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (SL9229R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining

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