MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.
Function:
E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.
Subunit:
Interacts with DIO2.
Subcellular Location:
Endoplasmic reticulum membrane; Multi pass membrane protein.
Tissue Specificity:
Present in brain (at protein level).
Post-translational modifications:
Auto-ubiquitinated, which results in proteasomal degradation.
Similarity:
Contains 1 RING-CH-type zinc finger.
SWISS:
O60337
Gene ID:
10299
Database links:
Entrez Gene: 10299 Human
Entrez Gene: 223455 Mouse
Entrez Gene: 294862 Rat
Omim: 613297 Human
SwissProt: O60337 Human
SwissProt: Q6ZQ89 Mouse
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (SL9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MARCH6) Polyclonal Antibody, Unconjugated (SL9340R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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