Rabbit Anti-MARCH9 antibody

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E3 ubiquitin protein ligase MARCH9; MARCH IX; Membrane associated RING CH protein IX; membrane associated ring finger (C3HC4) 9; Membrane associated RING finger protein 9; RING finger protein 179; RNF179; MARH9_HUMAN.

Cat:

SL9343R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human MARCH9:201-300/346

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7 (human)cell Lysate at 40 ugPrimary: Anti- MARCH9(SL9343R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38kDObserved band size: 38 kDBlank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH9 (membrane-associated ring finger (C3HC4) 9), also known as RNF179, is a 346 amino acid multi-pass membrane protein that localizes to the golgi apparatus and contains one RING-CH-type zinc finger. Expressed ubiquitously, MARCH9 exists as a homodimer and functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to promote the degradation of target proteins, such as CD4 and MHSLCI. Multiple isoforms of MARCH9 exist due to alternative splicing events.

Function:
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHSLCI, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Subunit:
Homodimer.

Subcellular Location:
Golgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.

Tissue Specificity:
Ubiquitously expressed.

Similarity:
Contains 1 RING-CH-type zinc finger.

SWISS:
Q86YJ5

Gene ID:
92979

Database links:

Entrez Gene: 92979 Human

Omim: 613336 Human

SwissProt: Q86YJ5 Human

Unigene: 632709 Human

Picture

Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).

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