Rabbit Anti-HSPBAP1 antibody

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27 KdA heat shock protein-associated protein 1; HBAP1_HUMAN; hspbap; HSPB1-associated protein 1; HSPBAP1; PASS1; Protein associated with small stress protein 1; protein associating with small stress protein.

Cat:

SL6986R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human HSPBAP1:151-250/488

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T Cell(human) Lysate at 40 ugLymph node (mouse) Lysate at 40 ugHepG2 Cell(human) Lysate at 40 ugTestis (mouse) Lysate at 40 ugPrimary: Anti- HSPBAP1(SL6986R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55kDObserved band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (SL6986R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Heat Shock Protein BAP1 (heat shock 27-kDa-associated protein 1, or HSPBAP1) inhibits the function of heat shock protein 27, which has a neuroprotective effect during experimentally induced epileptic neuropathology. The HSPBAP1 protein, also known as PASS1, contains a jmjC motif, suggesting it is a member of the jumonji family of transcription factors. There is evidence of domain swapping within the jumonji family genes, and the HSPBAP1 gene has been shown to fuse with a novel gene called DIRC3 in hereditary renal cell cancer.

Function:
May play a role in cellular stress response.

Subunit:
Interacts with CRYAB and HSPB1.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Widely expressed.

DISEASE:
Note=A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma. Translocation t(2;3)(q35;q21) with the putative pseudogene DIRC3. Produces an hybrid mRNA encoding a truncated HSPBAP1 lacking the first 36 amino acids.

Similarity:
Contains 1 JmjC domain.

SWISS:
Q96EW2

Gene ID:
79663

Database links:

Entrez Gene: 79663 Human

Omim: 608263 Human

SwissProt: Q96EW2 Human

Unigene: 29169 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (SL6986R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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