Home > Product > Antibody > Rabbit Anti-FRA10AC1 antibody
Chromosome 10 open reading frame 4; F26C11.1 like; C10orf4; FRA10AC1 protein; Fragile site 10q23.3; PRO2972; Protein FRA10AC1; Rare folic acid type fragile site FRA(10)(q23.3) candidate gene 1; F10C1_HUMAN.
Cat:
SL9767R
Species Reactivity:
Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human C10orf4:101-200/315
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: 293T(Human) Cell Lysate at 40 ugPrimary: Anti-C10orf4   (SL9767R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C10orf4) Polyclonal Antibody, Unconjugated (SL9767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
Price: $
Product PDFs
Datasheet:


The protein encoded by this gene is a nuclear phosphoprotein of unknown function. This gene contains a tandem CGG repeat region within a CpG island that normally consists of 8-14 repeats but can expand to over 200 repeats. The repeat region is within the 5' UTR of some transcript variants, but is intronic to another variant. The expanded repeat allele is a fragile site and becomes hypermethylated, causing a reduction in gene expression. A disease phenotype has not been associated with expanded alleles. This gene is found within the rare FRA10A folate-sensitive fragile site. [provided by RefSeq, Dec 2016]

Subcellular Location:
Nuclear.

Tissue Specificity:
Ubiquitously expressed with higher expression in brain, heart, skeletal muscle, kidney and liver.

SWISS:
Q70Z53

Gene ID:
118924

Database links:


  • Swiss-Prot: Q70Z53   human
  • Entrez Gene: 118924  human




Picture

Sample:
293T(Human) Cell Lysate at 40 ug
Primary: Anti-C10orf4   (SL9767R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C10orf4) Polyclonal Antibody, Unconjugated (SL9767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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