Rabbit Anti-ELYS antibody | Sunlong Medical

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ahctf1; AT hook containing transcription factor 1; DKFZp434N093; ELYS; AT-hook-containing transcription factor 1; ELYS transcription factor like protein TMBS62; ELYS_HUMAN; Embryonic large molecule derived from yolk sac; MST108; MSTP108; Protein ELYS; Pro

Cat:

SL9176R

Species Reactivity：

Mouse,(predicted: Human,Rat,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ELYS:451-550/2266

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spinal cord (Mouse) Lysate at 40 ugPrimary: Anti- ELYS (SL9176R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 252 kDObserved band size: 252 kDParaformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELYS) Polyclonal Antibody, Unconjugated (SL9176R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ELYS is a protein of the nuclear matrix that contains one AT hook DNA-binding domain. ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division. Nuclear pores span the nuclear envelope and act as gated aqueous channels to regulate the transport of macromolecules between the nucleus and cytoplasm, from individual proteins and RNAs to entire viral genomes. The A.T. hook near the SLCterminus of likely indicates ELYS can function as a transcription factor which may play a specific role in hematopoietic tissues. It also may play a role in early embryo development, as ELYS is critical for the survival of inner cells of the blastocyst.

Function:
Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.

Subunit:
Associates with the Nup107-160 subcomplex of the NPC.

Subcellular Location:
Cytoplasm. Nucleus matrix. Chromosome; centromere; kinetochore. Nucleus; nucleoplasm. Nucleus; nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase.

Similarity:
Belongs to the ELYS family.
Contains 1 A.T hook DNA-binding domain.

SWISS:
Q8WYP5

Gene ID:
25909

Database links:

Entrez Gene: 25909 Human

Omim: 610853 Human

SwissProt: Q8IZA4 Human

SwissProt: Q8WYP5 Human

Unigene: 300887 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELYS) Polyclonal Antibody, Unconjugated (SL9176R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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