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Home > Product > Antibody > Rabbit Anti-WDFY3 antibody
Alfy; Autophagy linked FYVE protein; KIAA0993; MGC16461; WD repeat and FYVE domain containing 3; ZFYVE25; WDFY3_HUMAN.
Cat:
SL9924R
Species Reactivity：
Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human WDFY3:951-1050/3526
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (SL9924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (SL9924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


WDFY3 was identified in a screen for phosphatidylinositol 3 phosphate binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein.

Subcellular Location:
Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.

Tissue Specificity:
Ubiquitous.

Similarity:
Contains 1 BEACH domain.
Contains 1 FYVE-type zinc finger.
Contains 5 WD repeats.

SWISS:
Q8IZQ1

Gene ID:
23001

Database links:

Entrez Gene: 23001 Human

SwissProt: Q8IZQ1 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (SL9924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (SL9924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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