Rabbit Anti-IRX5 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-IRX5 antibody

Homeodomain protein IRX-2A; Homeodomain Protein IRXB2; Iroquois Homeobox Protein 5; Iroquois-class homeodomain protein IRX-5; IRX2A; Irx5; IRX5_HUMAN; IRXB2.

Cat:

SL9469R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human IRX5:151-250/483

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebellum (Mouse) Lysate at 40 ugPrimary: Anti-IRX5 (SL9469R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRX5) Polyclonal Antibody, Unconjugated (SL9469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Establishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina (By similarity). Could be involved in the regulation of both the cell cycle and apoptosis in prostate cancer cells.

Function:
Establishes the cardiac repolarization gradient by itsrepressive actions on the KCND2 potassium-channel gene. Requiredfor retinal cone bipolar cell differentiation. May regulatecontrast adaptation in the retina and control specific aspects ofvisual function in circuits of the mammalian retina (Bysimilarity). Could be involved in the regulation of both the cellcycle and apoptosis in prostate cancer cells. Involved incraniofacial and gonadal development. Modulates the migration ofprogenitor cell populations in branchial arches and gonads byrepressing CXCL12.

Subcellular Location:
Nucleus.

DISEASE:
Defects in IRX5 are the cause of Hamamy syndrome (HMMS)[MIM:611174]. A sydrome characterized by severe hypertelorism,upslanting palpebral fissures, brachycephaly, abnormal ears,sloping shoulders, enamel hypoplasia, and osteopenia with repeatedfractures. Additional features include myopia, mild to moderatesensorineural hearing loss, gonadal anomalies, and borderlineintelligence.

Similarity:
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
A2RRB5

Gene ID:
10265

Database links:

Entrez Gene: 10265 Human

Entrez Gene: 54352 Mouse

Entrez Gene: 498918 Rat

Omim: 606195 Human

SwissProt: A2RRB5 Human

SwissProt: P78411 Human

SwissProt: Q9JKQ4 Mouse

Unigene: 435730 Human

Unigene: 101153 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRX5) Polyclonal Antibody, Unconjugated (SL9469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall