Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF828, is a 812 amino acid protein that contains one C2H2-type zinc finger and is localized to the cytoplasm and the nucleus. The gene encoding ZNF828 maps to chromosome 13. Comprising nearly 4% of the human genome, chromosome 13 contains around 114 million base pairs and encodes over 400 genes. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.
Function:
Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Subunit:
Interacts with MAD2L2. Interacts with POGZ, CBX1, CBX3 and CBX5.
Subcellular Location:
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.
Post-translational modifications:
Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
Similarity:
Contains 1 C2H2-type zinc finger.
SWISS:
Q96JM3
Gene ID:
283489
Database links:
Entrez Gene: 283489 Human
Entrez Gene: 101994 Mouse
Entrez Gene: 306647 Rat
SwissProt: Q96JM3 Human
SwissProt: Q8K327 Mouse
Unigene: 7542 Human
Unigene: 289346 Mouse
Unigene: 412576 Mouse
Unigene: 3851 Rat
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Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF828) Polyclonal Antibody, Unconjugated (SL9607R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF828) Polyclonal Antibody, Unconjugated (SL9607R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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