Rabbit Anti-SLC30A3 antibody

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SLC30A3; Solute carrier family 30 (zinc transporter), member 3; Solute carrier family 30 member 3; Zinc transporter 3; Zinc transporter ZnT 3; ZnT-3; ZNT3; ZNT3_HUMAN; OTTHUMP00000201041.

Cat:

SL8717R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human SLC30A3/ZNT3:21-120/388

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Positive sample：Paraformaldehyde-fixed, paraffin embedded (mouse brain);Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat intestine);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC30A3) Polyclonal Antibody, Unconjugated (SL8717R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SLC30A3 Polyclonal Antibody, Unconjugated(SL8717R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Zinc, an essential element required for cell proliferation and differentiation, plays a role in a diverse array of cellular functions, including acting as a cofactor for numerous enzymes and transcription factors and as a neuroregulator. The zinc transporter (ZnT) family regulates the supply of zinc within cells, and its members are characterized by containing six membrane-spanning domains, a large histidine-rich intracellular loop, and a SLCterminal tail. ZnT-3 mediates the uptake of zinc into vesicles in brain and testis.

Function:
Involved in accumulation of zinc in synaptic vesicles.

Subunit:
Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein (Probable).

Similarity:
Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.

SWISS:
Q99726

Gene ID:
7781

Database links:

Entrez Gene: 7781 Human

Entrez Gene: 22784 Mouse

Entrez Gene: 366568 Rat

Omim: 602878 Human

SwissProt: Q99726 Human

SwissProt: P97441 Mouse

SwissProt: Q6QIX3 Rat

Unigene: 467981 Human

Unigene: 1396 Mouse

Unigene: 9297 Rat

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SLC30A3 Polyclonal Antibody, Unconjugated(SL8717R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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