Rabbit Anti-IRE1 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-IRE1 antibody

IRE1a; Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requirin

Cat:

SL8136R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human IRE1a:252-260/977

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/testICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (SL8136R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: Raji. Primary Antibody (green line): Rabbit Anti-IRE1 antibody (SL8136R ) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new SLCterminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Subunit:
Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.

Subcellular Location:
Endoplasmic reticulum membrane.

Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.

SWISS:
O7592

Gene ID:
2081

Database links:

Entrez Gene: 2081 Human

Entrez Gene: 78943 Mouse

Entrez Gene: 419613 Rat

Omim: 604033 Human

SwissProt: O7592 Human

SwissProt: Q9EQY0 Mouse

Unigene: 133982 Human

Unigene: 592041 Human

Unigene: 700027 Human

Unigene: 20452 Mouse

Unigene: 340943 Mouse

Unigene: 226435 Rat

Picture

Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-IRE1 antibody (SL8136R )
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (17) | SL8136R has been referenced in 17 publications.

[IF=5.793] Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16 WB ; Human.

PubMed:33070776

[IF=2.791] Yang S et al. Induction of the unfolded protein response (UPR) during pseudorabies virus infection. Vet Microbiol. 2019 Dec;239:108485. WB ; Pig.

PubMed:31767094

[IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167. WB ; Human.

PubMed:29306025

[IF=1.664] Kishi,et al.Pro-metastatic signaling of the trans fatty acid elaidic acid is associated with lipid rafts.(2018) Oncology Letters. 15:4423-4426. WB ; Mouse.

PubMed:29556289

[IF=4.07] He, Wencheng, et al. "CSFV Infection Up-Regulates the Unfolded Protein Response to Promote Its Replication." Frontiers in Microbiology 8 (2017): 2129. WB ; Pig.

PubMed:29163417

[IF=3.29] Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176. WB ; Human.

PubMed:10.1039/c6ra26046b

[IF=3.28] Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13. WB ; Mouse.

PubMed:28070709

[IF=4.86] Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226. WB ; Human.

PubMed:26864693

[IF=3.12] Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014). WB ; Mouse.

PubMed:24699224

[IF=3.457] Lu, Y. et al. Protective effects of Danzhi jiangtang capsule on vascular endothelial damages induced by high-fat diet and palmitic acid. (2018) Biomedicine & Pharmacotherapy, 107, 1631–1128. WB ; Rat.

PubMed:30257381

[IF=2.092] Zhang Hao. et al. Protective effects of pterostilbene against hepatic damage, redox imbalance, mitochondrial dysfunction, and endoplasmic reticulum stress in weanling piglets. J Anim Sci. 2020 Oct;98(10): WB ; Pig.

PubMed:33027517

[IF=4.294] Mei Zhang. et al. Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Neurotoxicology. 2022 Jan;88:187 WB ; Human.

PubMed:34813867

[IF=3.038] Jiajia Chenet al. Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. Neurochem Res . 2020 Oct;45(10):2302-2311. WB, IHC ; rat.

PubMed:32681444

[IF=2.638] Yu-Dong Wei. et al. Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zool Res. 2021 Jan 18; 42(1): 14–27 WB ; Goat.

PubMed:33420764

[IF=4.868] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. WB ; Mouse.

PubMed:30728884

[IF=2.776] He Q et al. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ Toxicol Pharmacol. 2018 Oct;63:6-15. WB ; Mouse.

PubMed:30114659

[IF=4.19] Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015). WB ; Human.

PubMed:25950987