Rabbit Anti-RNF39 antibody | Sunlong Medical

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HZF; HZFW; HZFW1; LIRF; Protein HZFw; RING finger protein 39; Rnf39; RNF39_HUMAN.

Cat:

SL11393R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Monkey,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF39:182-230/420

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Protein: Raji lysate at 40ug; Primary: rabbit Anti-RNF39 (SL11393R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size: 38/45 kDObserved band size: 38 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF39) Polyclonal Antibody, Unconjugated (SL11393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF39 (RING finger protein 39), also known as HZFW, HZF or LIRF, is a 420 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger and one SPRY domain. Expressed in testis, RNF39 is thought to play a role in maintaining prolonged LTP (long term-potentiation, or the process by which synaptic strength continues to increases following chemical stimulation). Via its ability to influence the length of the LTP response, RNF39 functions to regulate early synaptic plasticity. Multiple isoforms of RNF39 exist due to alternative splicing events.

Function:
May play a role in prolonged long term-potentiation (LTP) maintenance.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed in testis.

Similarity:
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

SWISS:
Q9H2S5

Gene ID:
80352

Database links:

Entrez Gene: 80352 Human

Entrez Gene: 171387 Rat

Omim: 607524 Human

SwissProt: Q9H2S5 Human

SwissProt: Q920M2 Rat

Unigene: 121178 Human

Unigene: 144662 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF39) Polyclonal Antibody, Unconjugated (SL11393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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