This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008]
Function:
Involved in axonal outgrowth and fasciculation.
Subunit:
Homodimer; difulfide-linked. May form heterodimers with FEZ1. Interacts with synaptotagmin.
Tissue Specificity:
Expressed in nonneural tissues, such as heart, lung, spleen, muscle, testis, placenta and melanocytes.
Similarity:
Belongs to the zygin family.
SWISS:
Q9UHY8
Gene ID:
9637
Database links:
Entrez Gene: 9637 Human
Entrez Gene: 225020 Mouse
Entrez Gene: 94269 Rat
SwissProt: Q76LN0 Human
SwissProt: Q9UHY8 Human
SwissProt: Q6TYB5 Mouse
SwissProt: P97578 Rat
Unigene: 258563 Human
Unigene: 236835 Mouse
Unigene: 8714 Rat
Picture |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- FEZ2 (SL11168R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (FEZ2) Polyclonal Antibody, Unconjugated (SL11168R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
|
|
|