Home > Product > Antibody > Rabbit Anti-FEZ2 antibody
Zygin2; Fasciculation and elongation protein zeta 2; Fasciculation and elongation protein zeta-2; FEZ 2; Fez2; FEZ2_HUMAN; Highly similar to zyginII; HUM3CL; MGC117372; Pre T/NK cell associated protein; Zygin 2; Zygin II; Zygin-2.
Cat:
SL11168R
Species Reactivity:
Mouse,Rat,(predicted: Human,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human FEZ2/Zygin2:165-240/353
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Cerebrum (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti- FEZ2 (SL11168R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 46 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (FEZ2) Polyclonal Antibody, Unconjugated (SL11168R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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Unit:
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Product PDFs
Datasheet:


This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008]

Function:
Involved in axonal outgrowth and fasciculation.

Subunit:
Homodimer; difulfide-linked. May form heterodimers with FEZ1. Interacts with synaptotagmin.

Tissue Specificity:
Expressed in nonneural tissues, such as heart, lung, spleen, muscle, testis, placenta and melanocytes.

Similarity:
Belongs to the zygin family.

SWISS:
Q9UHY8

Gene ID:
9637

Database links:

Entrez Gene: 9637 Human

Entrez Gene: 225020 Mouse

Entrez Gene: 94269 Rat

SwissProt: Q76LN0 Human

SwissProt: Q9UHY8 Human

SwissProt: Q6TYB5 Mouse

SwissProt: P97578 Rat

Unigene: 258563 Human

Unigene: 236835 Mouse

Unigene: 8714 Rat



Picture

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- FEZ2 (SL11168R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (FEZ2) Polyclonal Antibody, Unconjugated (SL11168R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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