Rabbit Anti-ZDHHC3 antibody

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DHHC-3; GABA-A receptor-associated membrane protein 1; Golgi-specific DHHC zinc finger protein; Gramp1; Palmitoyltransferase ZDHHC3; Protein DHHC1; ZDHC3_HUMAN; Zdhhc3; Zinc finger DHHC domain-containing protein 3; Zinc finger protein 373; ZNF373.

Cat:

SL12034R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GODZ/ZNF373:201-299/299

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Lane 3: Human HL60 cell lysates Primary: Anti-GODZ/ZDHHC3 (SL12034R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDParaformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GODZ) Polyclonal Antibody, Unconjugated (SL12034R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Golgi-specific DHHC (Asp-His-His-Cys) zinc finger protein (GODZ), also known as, Palmitoyltransferase ZDHHC3 or Zinc finger protein 373, is a 327 amino acid protein member of the DHHC palmitoyltransferase family. Localized to the Golgi apparatus membrane, GODZ contains one DHHSLCtype zinc finger, which is necessary for its palmitoyltransferase activity. GODZ has been implicated in the palmitoylation and regulated trafficking of diverse substrates that function various inhibitory and excitatory synapses. Specifically, it palmitoylates the gamma subunit 2 of GABA(A) receptors, which leads to normal synaptic GABAergic inhibitory function. GODZ also palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. Two isoforms of GODZ exist as a result of alternative splicing events.

Function:
Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.

Subcellular Location:
Golgi apparatus membrane.

Post-translational modifications:
Autopalmitoylated.

Similarity:
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHSLCtype zinc finger.

SWISS:
Q9NYG2

Gene ID:
51304

Database links:

Entrez Gene: 51304 Human

Entrez Gene: 69035 Mouse

Entrez Gene: 301081 Rat

SwissProt: Q9NYG2 Human

SwissProt: Q8R173 Mouse

Unigene: 61430 Human

Unigene: 28300 Mouse

Unigene: 8573 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GODZ) Polyclonal Antibody, Unconjugated (SL12034R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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