Rabbit Anti-ZNF342 antibody

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zinc finger protein 342; Zinc finger protein 296; ZNF296; ZN296_HUMAN.

Cat:

SL12212R

Species Reactivity：

Human,Mouse,(predicted: Rat,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from Human ZNF342/ZNF296:231-350/475

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-ZNF342 (SL12212R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kDTissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ZNF342 Polyclonal Antibody, Unconjugated(SL12212R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the Krüppel C2H2-type zinc-finger protein family, ZNF342 (zinc finger protein 342), also known as Zinc finger protein 296, is a 475 amino acid nuclear protein that contains six C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation.

Function:
ZNF342 contains 6 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. It may be involved in transcriptional regulation.

Subcellular Location:
Nuclear

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.

SWISS:
Q8WUU4

Gene ID:
162979

Database links:

Entrez Gene: 162979 Human

Omim: 613226 Human

SwissProt: Q8WUU4 Human

Unigene: 192237 Human

Picture

Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ZNF342 Polyclonal Antibody, Unconjugated(SL12212R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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