OAZ is a 30-zinc finger, DNA-binding factor that associates with members of the Smad family of transcription factors in response to BMP2 activation. Bone morphogenic proteins (BMPs), are the largest group within the TGF Beta growth factors superfamily and are involved in embryonic development, specifically the formation of left-right asymmetry, neurogenesis, organogenesis and skeletal development. BMPs bind to surface receptors, which then phosphorylate serine residues of specific Smad proteins to induce Smad translocation to the nucleus and transcriptional activation of BMP targeted genes. OAZ specifically cooperates with the BMP-activated Smads, namely Smad1, 5 and 8, in binding to the CAGAC and TGGAGC boxes within the BRE, or BMP response element, and activating transcription. OAZ contains a BMP signaling module formed by two clusters of fingers that individually associate with either the Smads or the BMP response element. Distinct regions of OAZ, separate from the modules involved in BMP regulation, also enable OAZ to function as a transcriptional partner of Olf-1/ESF in olfactory epithelium and lymphocyte development, indicating that, as a multi–zinc finger protein, OAZ may have dual roles in signal transduction during development.
Function:
Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with ESF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing ESF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
Subunit:
Homodimer (By similarity). Interacts with ESF1 (By similarity). Interacts with SMAD1 and SMAD4. Interacts with PARP1. Interacts with CEP290.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum.
DISEASE:
Note=Defects in ZNF423 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. ZNF423 mutations have been found in patients with nephronophthisis, cerebellar vermis hypoplasia and situs inversus, and Joubert syndrome.
Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 30 C2H2-type zinc fingers.
SWISS:
Q2M1K9
Gene ID:
23090
Database links:
Entrez Gene: 23090 Human
Entrez Gene: 94187 Mouse
Entrez Gene: 94188 Rat
Entrez Gene: 566696 Zebrafish
Omim: 604557 Human
SwissProt: Q2M1K9 Human
SwissProt: Q80TS5 Mouse
SwissProt: O08961 Rat
SwissProt: A1L1R6 Zebrafish
Unigene: 530930 Human
Unigene: 23452 Mouse
Unigene: 472447 Mouse
Unigene: 9981 Rat
Unigene: 132594 Zebrafish
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ZNF423 Polyclonal Antibody, Unconjugated(SL12220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ZNF423/OAZ Polyclonal Antibody, Unconjugated(SL12220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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