Rabbit Anti-OVOL2 antibody | Sunlong Medical

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bA504H3.3; EUROIMAGE566589; hOvo 2; hOvo2; Ovo like 2 (Drosophila); Ovo like 2; OVOL 2; Transcription factor Ovo like 2; Zinc finger protein 339; ZNF 339; ZNF339; OVOL2_HUMAN.

Cat:

SL12274R

Species Reactivity：

Mouse,Rat,(predicted: Human,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human OVOL2:75-180/275

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Heart (Mouse) Lysate at 40 ugPrimary: Anti-OVOL2 (SL12274R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDParaformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OVOL2) Polyclonal Antibody, Unconjugated (SL12274R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Ovo family of zinc-finger transcription factors encode evolutionarily conserved genes including those from Caenorhabditis elegans, Drosophila melanogaster, mouse and human. Members of the Ovo family include Ovol1 and Ovol2. Ovol1 acts as a transcriptional repressor by interacting with key developmental signaling pathways such as Wnt and TGF-∫/BMP. Specifically, Ovol1 represses c-Myc and Id2 genes and establishes a balance between proliferation and differentiation of progenitor cells. Deletion of Ovol1 in mice leads to germ cell degeneration and defective sperm production in adult males. Ovol1 has also been shown to repress itself as well as Ovol2, which is thought to regulate neural development and vascular angiogenesis during embryogenesis.

Function:
OVOL2 (Ovo like 2) contains 4 C2H2 type zinc fingers. It belongs to the krueppel C2H2 type zinc finger protein family. It is a DNA binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. It probably acts as a transcription regulator.

Subcellular Location:
Nuclear.

Tissue Specificity:
Expressed in testis, ovary, heart and skeletal muscle.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.

SWISS:
Q9BRP0

Gene ID:
58495

Database links:

Entrez Gene: 58495 Human

Entrez Gene: 107586 Mouse

Entrez Gene: 296201 Rat

SwissProt: Q9BRP0 Human

SwissProt: Q8CIV7 Mouse

Unigene: 661013 Human

Unigene: 710157 Human

Unigene: 252750 Mouse

Unigene: 104639 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OVOL2) Polyclonal Antibody, Unconjugated (SL12274R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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