Rabbit Anti-EPS15R antibody

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epidermal growth factor receptor pathway substrate 15- ike 1; Epidermal growth factor receptor substrate 15 like 1; epidermal growth factor receptor substrate EPS15R; Eps15 related protein; eps15R;EP15R_HUMAN.

Cat:

SL1620R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human EPS15R:275-380/864

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-EPS15R Polyclonal Antibody, Unconjugated(SL1620R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-EPS15R Polyclonal Antibody, Unconjugated(SL1620R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation, as miscues in RTK signaling can result in cellular transformation events that may ultimately lead to cancer. Eps15 (EGF-receptor pathway substrate 15) is an EGF receptor substrate that become become tyrosine phosphorylated subsequent to EGF stimulation. Over expression of Eps15 in NIH/3T3 cells causes cellular transformation, implying involvement of Eps15 in the regulation of cell proliferation. Eps15R (Eps15-related), also known as Eps15L1 (epidermal growth factor receptor substrate 15-like 1), is an 864 amino acid protein that contains one EF-hand domain and three EH domains. Localized to a variety of places within the cell, including the nucleus, cytoplasm and membrane-coated pits, Eps15R is thought to function as a component of clathrin-coated pits. Like Eps15, Eps15R may play an important role in receptor-mediated endocytosis.

Function:
EPS15 and EPS15R are substrates of the epidermal growth factor (EGF) receptor kinase, which are characterized by their ability to bind to the clathrin adaptor protein complex adaptor protein 2, and the presence of a protein:protein interaction domain, the EH domain. Evidence suggests that EPS15 and EPS15R are involved in endocytosis. The major difference between these two related proteins is that EPS15R is also found in the nucleus, whereas EPS15 is excluded from this compartment at steady state.

Subunit:
Interacts with EPS15, AGFG1/HRB and AGFG2/HRBL. Associates with the clathrin-associated adapter protein complex 2 (AP-2) (By similarity). Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2.

Subcellular Location:
Cell Membrane and Nuclear.

Post-translational modifications:
Phosphorylated on tyrosine residues by EGFR (By similarity).

Similarity:
Contains 1 EF-hand domain.
Contains 3 EH domains.

SWISS:
Q9UBC2

Gene ID:
58513

Database links:

Entrez Gene: 58513 Human

Omim: 600051 Human

SwissProt: Q9UBC2 Human

Unigene: 654639 Human

Unigene: 744842 Human

Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-EPS15R Polyclonal Antibody, Unconjugated(SL1620R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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