Rabbit Anti-phospho-ETS1 (Thr38) antibody

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ETS1 (phospho T38); ETS1 (phospho Thr38); p-ETS1 (phospho T38); Avian erythroblastosis virus E26 (v ets) oncogene homolog 1; C ets 1 protein; ETS 1; Ets protein; ETS1; ETS-1; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; p54; Protein C-ets-1; v ets a

Cat:

SL13113R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human ETS1 around the phosphorylation site of Thr38:LL(p-T)PS

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2(Human) Cell Lysate at 30 ugSpleen (Mouse) Lysate at 40 ugLymph node (Mouse) Lysate at 40 ugJurkat(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-ETS1 (Thr38) (SL13113R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 50 kDParaformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ETS1 (Thr38)) Polyclonal Antibody, Unconjugated (SL13113R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011].

Function:
Transcription factor.

Subunit:
Interacts with MAF and MAFB. Interacts with PAX5, the interaction alters DNA-binding properties. Binds to DAXX. Interacts with UBE2I.

Subcellular Location:
Nucleus.

Post-translational modifications:
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.

Similarity:
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.

SWISS:
P14921

Gene ID:
2113

Database links:
UniProtKB/Swiss-Prot: P14921.1

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ETS1 (Thr38)) Polyclonal Antibody, Unconjugated (SL13113R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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