Rabbit Anti-C4orf21 antibody

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Chromosome 4 open reading frame 21; Uncharacterized protein C4orf21; Uncharacterized protein FLJ44066; YD002_HUMAN.

Cat:

SL15188R

Species Reactivity：

Human,Mouse,(predicted: Rat,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human C4orf21:101-200/926

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 40 ugMolt-4 (Human) Cell Lysate at 30 ugHL60 (Human) Cell Lysate at 30 ugPrimary: Anti- C4orf21 (SL15188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 119 kDObserved band size: 119 kDTissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-C4orf21 Polyclonal Antibody, Unconjugated(SL15188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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C4orf12 (chromosome 4 open reading frame 12) is a 926 amino acid single-pass membrane protein that exists as two isoforms. The gene encoding C4orf21 maps to human chromosome 4. Human chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded by a gene that is located on chromosome 4, and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

Subcellular Location:
Membrane; Single-pass membrane protein (Potential).

SWISS:
Q86YA3

Gene ID:
55345

Database links:

Entrez Gene: 55345 Human

Entrez Gene: 71643 Mouse

SwissProt: Q6ZU11 Human

SwissProt: Q86YA3 Human

SwissProt: Q0VGT4 Mouse

Unigene: 380346 Human

Picture

Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-C4orf21 Polyclonal Antibody, Unconjugated(SL15188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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