The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin SLCterminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker.
Function:
Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
Subunit:
Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recritment to the SAGA complex.
Subcellular Location:
Nucleus.
Tissue Specificity:
Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
Similarity:
Belongs to the peptidase C19 family. UBP8 subfamily.
Contains 1 UBP-type zinc finger.
SWISS:
Q9UPT9
Gene ID:
23326
Database links:
Entrez Gene: 23326 Human
Entrez Gene: 216825 Mouse
Entrez Gene: 30641 Rat
Omim: 612116 Human
SwissProt: Q9UPT9 Human
SwissProt: Q5DU02 Mouse
Unigene: 462492 Human
Unigene: 30602 Mouse
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Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-USP22 (SL8611R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 63 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-USP22 Polyclonal Antibody, Unconjugated(SL8611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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