Rabbit Anti-GPS2 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-GPS2 antibody

AMF-1; G protein pathway suppressor 2; GPS-2; GPS2; MGC104294; MGC119287; MGC119288; MGC119289; GPS2_HUMAN.

Cat:

SL15395R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GPS2:1-100/327

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Rat Kidney tissue lysatesPrimary: Anti-GPS2 (SL15395R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDaObserved band size: 37 kDaSample:Testis (Mouse) Lysate at 40 ugPrimary: Anti- GPS2 (SL15395R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37kDObserved band size: 37kDBlank control: A549. Primary Antibody (green line): Rabbit Anti-GPS2 antibody (SL15395R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq, Jul 2008]

Function:
Suppresses G-protein- and mitogen-activated protein kinase-mediated signal transduction.

Subunit:
Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2.

Tissue Specificity:
Widely expressed.

SWISS:
Q13227

Gene ID:
2874

Database links:

Entrez Gene: 2874 Human

SwissProt: Q13227 Human

Unigene: 438219 Human

Picture

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- GPS2 (SL15395R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 37kD

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-GPS2 antibody (SL15395R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall