Rabbit Anti-MYCBPAP antibody

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AMAM-1; AMAP-1; AMAP1; AMY-1-binding protein 1; DKFZp434N1415; MYBPP_HUMAN; MYCBP-associated protein; Mycbpap.

Cat:

SL13614R

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human MYCBPAP:401-500/947

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYCBPAP) Polyclonal Antibody, Unconjugated (SL13614R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYCBPAP) Polyclonal Antibody, Unconjugated (SL13614R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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AMAP-1 (AMY-1-binding protein 1), also known as AMAM-1 or MYCBPAP (MYCBP associated protein), is a 947 amino acid protein that is expressed specifically in testis and is involved in spermatogenesis and synaptic processes. AMAP-1 colocalizes with MYCBP (AMY-1) in cytoplasm and also localizes to membrane. The gene encoding AMAP-1 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

Function:
May play a role in spermatogenesis. May be involved in synaptic processes.

Subcellular Location:
Cytoplasm. Membrane. Colocalizes with MYCBP in the cytoplasm.

Tissue Specificity:
Expressed specifically in testis.

SWISS:
Q8TBZ2

Gene ID:
16873

Database links:

Entrez Gene: 16873 Human

Omim: 609835 Human

SwissProt: Q8TBZ2 Human

Unigene: 398196 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYCBPAP) Polyclonal Antibody, Unconjugated (SL13614R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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