Rabbit Anti-HSH2D antibody | Sunlong Medical

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Adaptor in lymphocytes of unknown function X; ALX; FLJ14886; hematopoietic SH2 domain containing; Hematopoietic SH2 domain containing protein; Hematopoietic SH2 domain-containing protein; Hematopoietic SH2 protein; HSH2; Hsh2d; HSH2D_HUMAN.

Cat:

SL13643R

Species Reactivity：

Rat,(predicted: Human,Mouse,Dog,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human HSH2D:21-120/352

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-HSH2D Polyclonal Antibody, Unconjugated(SL13643R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-HSH2D Polyclonal Antibody, Unconjugated(SL13643R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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HSH2 is a 352 amino acid nuclear and cytoplasmic protein that is predominantly expressed in spleen and hematopoietic cells, such as peripheral blood leukocytes, and weakly expressed in prostate, thymus, heart, small intestine and placenta. Containing an SH2 domain, four PXXP polyproline sequences and two possible tyrosine phosphorylation sites, HSH2 interacts with tyrosine kinases Fes and ACK. Considered an adaptor protein, HSH2 participates in tyrosine kinase signaling and may be involved in the regulation of cytokine signaling and cytoskeletal reorganization in hematopoietic cells. HSH2 may also act to attenuate apoptosis through modulating the apoptotic response by promoting mitochondrial stability. HSH2 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 19p13.11.

Function:
May be a modulator of the apoptotic response through its ability to affect mitochondrial stability (By similarity). Adapter protein involved in tyrosine kinase and CD28 signaling. Seems to affect CD28-mediated activation of the RE/AP element of the interleukin-2 promoter.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Predominantly expressed in spleen and hematopoietic cells such as peripheral blood leukocytes and weakly expressed in prostate, thymus, heart, small intestine and placenta.

Post-translational modifications:
May be phosphorylated by FES and ACK1.

Similarity:
Contains 1 SH2 domain.

SWISS:
Q96JZ2

Gene ID:
84941

Database links:

Entrez Gene: 84941 Human

Omim: 608349 Human

SwissProt: Q96JZ2 Human

Unigene: 631617 Human

Picture

Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-HSH2D Polyclonal Antibody, Unconjugated(SL13643R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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