The ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of ARF-dependent pathways. This family includes ASAP1, ACAP1, ACAP2, AGAP1, ARAP1, ARAP2, Centaurin a1, Centaurin g3 and the recently discovered Centaurin a2. Expressed in a wide variety of tissues such as fat, heart and skeletal muscle, Centaurin a2 is thought to negatively regulate ARF-mediated actin rearrangement by binding activated PI 3-kinase. Although the exact function of Centaurin a2 is not yet known, its high sequence similarity with Centaurin a1 suggests that it may also act as an ARF6 GTPase.
Function:
GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity.
Subcellular Location:
Cytoplasm. Cell membrane. Constitutively associated with the plasma membrane. Excluded from the nucleus.
Tissue Specificity:
Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow.
Similarity:
Contains 1 Arf-GAP domain.
Contains 2 PH domains.
SWISS:
Q9NPF8
Gene ID:
55803
Database links:
Entrez Gene: 55803 Human
Entrez Gene: 216991 Mouse
Entrez Gene: 56826 Rat
SwissProt: Q9NPF8 Human
SwissProt: Q8R2V5 Mouse
SwissProt: Q9JK15 Rat
Unigene: 514063 Human
Unigene: 341520 Mouse
Unigene: 203877 Rat
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Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-Centaurin alpha 2 (SL13841R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44kD
Observed band size: 44kD
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Centaurin alpha 2) Polyclonal Antibody, Unconjugated (SL13841R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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