Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]
Function:
Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.
Subcellular Location:
Cell Membrane and Mitochondrial
Similarity:
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase SLCterminal domain.
Contains 1 phosphagen kinase N-terminal domain.
SWISS:
P12532
Gene ID:
1159
Database links:
Entrez Gene: 1159 Human
Entrez Gene: 281692 Cow
Entrez Gene: 548596 Human
Entrez Gene: 12716 Mouse
Entrez Gene: 29593 Rat
Omim: 123290 Human
SwissProt: Q9TTK8 Cow
SwissProt: P12532 Human
SwissProt: P30275 Mouse
SwissProt: Q29577 Pig
SwissProt: P25809 Rat
SwissProt: Q7ZUN7 Zebrafish
Unigene: 425633 Human
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Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Creatine kinase MT (SL14050R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Creatine kinase MT) Polyclonal Antibody, Unconjugated (SL14050R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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