Rabbit Anti-CRISP11 antibody

Home > Product > Antibody > Rabbit Anti-CRISP11 antibody

CRISP-11; CRISPLD2; CRISP11; Crispld2; CRLD2_HUMAN; Cysteine-rich secretory protein 11; Cysteine-rich secretory protein LCCL domain-containing 2; DKFZP434B044; LCCL domain-containing cysteine-rich secretory protein 2; LCRISP2; LCRISP2; MGC74865.

Cat:

SL14061R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human CRISP11:101-200/497

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: pancreas (Mouse) Lysate at 40 ugPrimary: Anti-CRISP11(SL14061R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDParaformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRISP11) Polyclonal Antibody, Unconjugated (SL14061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Cysteine-rich secretory proteins (CRISPs) represent a family of evolutionarily conserved proteins that may play a role in the innate immune system and are transcriptionally regulated by androgens in several tissues. CRISP proteins are highly expressed in the mammalian reproductive tract and in the venom secretory ducts of some reptiles. CRISP-11 (cysteine-rich secretory protein 11), also known as cysteine-rich secretory protein LCCL domain-containing 2 (CRISPLD2 or LCRISP2), is a 497 amino acid protein containing 2 LCCL domains, which are thought to function as autonomous folding domains used to construct modular proteins through exon shuffling. Serum concentrations of CRISP-11 have been shown to be an indicator of a patient’s exposure to lipopolysaccahride (LPS), the immunostimulatory component of Gram-negative bacteria, and can determine one’s sensitivity to it.

Function:
Promotes matrix assembly.

Subcellular Location:
Secreted.

Similarity:
Belongs to the CRISP family.
Contains 2 LCCL domains.

SWISS:
Q9H0B8

Gene ID:
83716

Database links:

Entrez Gene: 83716 Human

Entrez Gene: 78892 Mouse

Entrez Gene: 171547 Rat

Omim: 612434 Human

SwissProt: Q9H0B8 Human

SwissProt: Q8BZQ2 Mouse

Unigene: 513779 Human

Unigene: 617091 Human

Unigene: 264136 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRISP11) Polyclonal Antibody, Unconjugated (SL14061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall