Rabbit Anti-GRF2 antibody | Sunlong Medical

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C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTH

Cat:

SL16311R

Species Reactivity：

Mouse,(predicted: Human,Rat,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GRF2:20-120/1237

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Placenta (Mouse) Lysate at 40 ugPrimary: Anti-GRF2 (SL16311R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 140 kDObserved band size: 120 kDSample: Lane 1: Human MCF-7 cell lysates Lane 2: Human HL-60 cell lysates Lane 3: Human JAR cell lysates Lane 4: Human HeLa cell lysates Primary: Anti-GRF2 (SL16311R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 140 kDaObserved band size: 140 kDa

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Function:
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.

Tissue Specificity:
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.

Similarity:
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.

SWISS:
O14827

Gene ID:
2889

Database links:

Entrez Gene: 2889 Human

Entrez Gene: 107746 Mouse

Omim: 600303 Human

SwissProt: Q13905 Human

Unigene: 127897 Human

Unigene: 298274 Mouse

Picture

Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HL-60 cell lysates
Lane 3: Human JAR cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-GRF2 (SL16311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa

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