Rabbit Anti-INSM2 antibody | Sunlong Medical

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IA 6; IA6; INSM2_HUMAN; Insulinoma associated 2; Insulinoma associated protein 2; mlt1; Zinc finger protein IA 6; Zinc finger protein IA6.

Cat:

SL16676R

Species Reactivity：

Human,Rat,(predicted: Mouse,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human INSM2:401-500/566

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: U937 Cell (Human) Lysate at 40 ugRaji Cell (Human) Lysate at 40 ugPrimary: Anti- INSM2 (SL16676R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58 kDObserved band size: 63 kDTissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-INSM2 Polyclonal Antibody, Unconjugated(SL16676R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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INSM2 is a 566 amino acid nuclear protein. INSM2 is believed to act as a growth or tumor suppressor in certain neurons and in liver cells. INSM2 contains five zinc finger C2H2-type domains which bind nucleic acids. Zinc fingers are composed of 25-30 amino acid residues and have the ability to bind about five nucleotides. C2H2-type zinc fingers have two cysteines on one extremity of the domain, and two histidines on the other extremity. These four cysteines and histidines interact with one zinc atom, resulting in the finger-like conformation of the domain. It is believed that the C2H2-type zinc fingers require the binding of a zinc atom in order for the finger to bind DNA or RNA.

Function:
INSM2 may function as a growth suppressor or tumor suppressor in liver cells and in certain neurons.

Subcellular Location:
Nuclear

Tissue Specificity:
Expressed in heart, liver, skeletal muscle, kidney and pancreas, and, to a lesser extent, in brain, lung and spleen. In the pancreas, expressed in islet cells, including insulin- and glucagon-producing alpha- and beta-cells, but not in acinar cells (at protein level). Detected in adrenal glands, particularly in the deeper layer of the cortex (at protein level). {ECO:0000269|PubMed:21343251}.

Similarity:
Contains 5 C2H2-type zinc fingers.

SWISS:
Q96T92

Gene ID:
84684

Database links:

Entrez Gene: 84684 Human

Entrez Gene: 56856 Mouse

Entrez Gene: 314131 Rat

SwissProt: Q96T92 Human

SwissProt: Q9JMC2 Mouse

Unigene: 62813 Human

Picture

Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-INSM2 Polyclonal Antibody, Unconjugated(SL16676R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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