Rabbit Anti-KDM6A antibody | Sunlong Medical

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bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX); bA386N14.2; bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX; DKFZp686A03225; Histone demethylase UTX; KABUK2; Kdm6a; KDM

Cat:

SL16948R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human KDM6A:661-750/1401

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KDM6A) Polyclonal Antibody, Unconjugated (SL16948R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KDM6A) Polyclonal Antibody, Unconjugated (SL16948R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit)（sp-0023）instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjSLCdomain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq, Nov 2009]

Function:
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.

Subunit:
Interacts with TLE1 (By similarity).

Subcellular Location:
Nucleus.

Similarity:
Belongs to the UTX family.
Contains 1 JmjC domain.
Contains 8 TPR repeats.

SWISS:
O15550

Gene ID:
7403

Database links:

Entrez Gene: 7403 Human

Entrez Gene: 22289 Mouse

Omim: 300128 Human

SwissProt: O15550 Human

SwissProt: O70546 Mouse

Unigene: 522616 Human

Unigene: 257498 Mouse

Unigene: 417709 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KDM6A) Polyclonal Antibody, Unconjugated (SL16948R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit)（sp-0023）instructionsand DAB staining.

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