Home > Product > Antibody > Rabbit Anti-KIAA1370 antibody
K1370_HUMAN; Kiaa1370; Uncharacterized protein KIAA1370.
Cat:
SL17002R
Species Reactivity:
Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Horse,Rabbit,)
Immunogen:
KLH conjugated synthetic peptide derived from human KIAA1370:761-860/1076
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample:Panc-1 (Human)Cell Lysate at 40 ugPrimary: Anti-KIAA1370(SL17002R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 121kDObserved band size: 121kDParaformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIAA1370) Polyclonal Antibody, Unconjugated (SL17002R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Sample:Panc-1 (Human)Cell Lysate at 40 ug
Primary: Anti-KIAA1370(SL17002R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 121kD
Observed band size: 121kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIAA1370) Polyclonal Antibody, Unconjugated (SL17002R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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