Rabbit Anti-TRIM68 antibody

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E3 ubiquitin-protein ligase TRIM68; FLJ10369; GC109; MGC126176; RING finger protein 137; RNF 137; RNF137; Ro/SSA1 related protein; SS 56; SS-56; SS56; SSA protein SS 56; SSA protein SS-56; TRI68_HUMAN; TRIM 68; Trim68; Tripartite motif con

Cat:

SL17123R

Species Reactivity：

Rat,(predicted: Human,Mouse,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TRIM68:201-300/485

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2(Human) Cell Lysate at 40 ugPrimary: Anti-TRIM68 (SL17123R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 56 kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM68) Polyclonal Antibody, Unconjugated (SL17123R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein which contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells. [provided by RefSeq, Oct 2011]

Function:
Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.

Subunit:
Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60.

Subcellular Location:
Cytoplasm > perinuclear region. Nucleus. Colocalized with AR in nucleus.

Tissue Specificity:
Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.

Post-translational modifications:
Auto-ubiquitinated.

Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

SWISS:
Q6AZZ1

Gene ID:
55128

Database links:

Entrez Gene: 55128 Human

Entrez Gene: 101700 Mouse

Omim: 613184 Human

SwissProt: Q6AZZ1 Human

SwissProt: Q8K243 Mouse

Unigene: 523438 Human

Unigene: 738828 Human

Unigene: 39043 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM68) Polyclonal Antibody, Unconjugated (SL17123R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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