Rabbit Anti-TRMT12 antibody

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Alpha amino alpha carboxypropyl transferase TYW2; FLJ20772; Homolog of yeast tRNA methyltransferase; Radical S-adenosyl methionine and flavodoxin domain-containing protein 2; TRM 12; TRM12; TRMT 12; TRMT12; tRNA methyltransferase 12; tRNA methylt

Cat:

SL17144R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human TRMT12:231-330/448

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Ovary (Mouse) Lysate at 40 ugPrimary: Anti- TRMT12-1124 (SL17144R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDParaformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRMT12) Polyclonal Antibody, Unconjugated (SL17144R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames. TRM12 (tRNA methyltransferase 12 homolog (S. cerevisiae)), also known as TYW2 (tRNA-yW-synthesizing protein 2) or TRMT12, is a 448 amino acid protein that belongs to the RNA methyltransferase trmD family and TYW2 subfamily. TRM12 is the human homolog of a yeast gene that is essential for the synthesis of yW (wybutosine), a guanosine that stabilizes codon-anticodon associations near the anticodon of phenylalanine tRNA during ribosomal decoding. The gene encoding TRRM12 maps to human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Function:
Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.

Similarity:
Belongs to the RNA methyltransferase trmD family. TYW2 subfamily.

SWISS:
Q53H54

Gene ID:
55039

Database links:

Entrez Gene: 55039 Human

Entrez Gene: 68260 Mouse

Omim: 611244 Human

SwissProt: Q53H54 Human

SwissProt: Q8BG71 Mouse

Unigene: 9925 Human

Unigene: 271765 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRMT12) Polyclonal Antibody, Unconjugated (SL17144R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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