Rabbit Anti-WASF3 antibody | Sunlong Medical

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KIAA0900; Protein WAVE-3; Protein WAVE3; SCAR3; Verprolin homology domain containing protein 3; Verprolin homology domain-containing protein 3; WASF3; WASF3_HUMAN; WASP family protein member 3; WAVE3; Wiskott Aldrich syndrome protein family member 3; Wisk

Cat:

SL10578R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human WASF3:21-120/502

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: ovary (Mouse) Lysate at 40 ugPrimary: Anti-WASF3(SL10578R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RWASF3) Polyclonal Antibody, Unconjugated (SL10578) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.

Function:
Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.

Subunit:
Binds actin and the Arp2/3 complex.

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Expressed in ovary and brain.

Post-translational modifications:
Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.

Similarity:
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.

SWISS:
Q9UPY6

Gene ID:
10810

Database links:

Entrez Gene: 10810 Human

Entrez Gene: 245176 Mouse

Omim: 605068 Human

SwissProt: Q9UPY6 Human

SwissProt: Q8VHI6 Mouse

Unigene: 635221 Human

Unigene: 472750 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RWASF3) Polyclonal Antibody, Unconjugated (SL10578) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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