Rabbit Anti-HSD17B13 antibody

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17-beta-HSD 13; 17-beta-hydroxysteroid dehydrogenase 13; DHB13_HUMAN; HMFN0376; Hsd17b13; SCDR9; Short-chain dehydrogenase/reductase 9; UNQ497/PRO1014.

Cat:

SL17402R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human HSD17B13:171-270/300

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-HSD17B13 (SL17402R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 34 kDSample: Lane 1: Mouse Kidney tissue lysates Lane 2: Rat Liver tissue lysates Lane 3: Rat Kidney tissue lysates Primary: Anti-HSD17B13 (SL17402R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDaObserved band size: 31 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD17B13) Polyclonal Antibody, Unconjugated (SL17402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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17 beta-HSD13 (17 beta hydroxysteroid dehydrogenase type 13), also designated Short-chain dehydrogenase/reductase 9 (SCDR9), belongs to the 17 beta-HSD family of proteins, which regulate the availability of steroids within various tissues throughout the body. 17 beta-HSD13 is a 300 amino acid secreted protein that is highly expressed in liver and is also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. The gene encoding 17 beta-HSD13 maps to chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

Function:
Highly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis.

Subcellular Location:
Secreted.

Similarity:
Belongs to the short-chain dehydrogenases/reductases (SDR) family.

SWISS:
Q7Z5P4

Gene ID:
345275

Database links:

Entrez Gene: 345275 Human

Entrez Gene: 243168 Mouse

Entrez Gene: 305150 Rat

Omim: 612127 Human

SwissProt: Q7Z5P4 Human

SwissProt: Q8VCR2 Mouse

SwissProt: Q5M875 Rat

Unigene: 284414 Human

Unigene: 284944 Mouse

Unigene: 25104 Rat

Picture

Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Rat Kidney tissue lysates
Primary: Anti-HSD17B13 (SL17402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 31 kDa

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD17B13) Polyclonal Antibody, Unconjugated (SL17402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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