Rabbit Anti-SPERT antibody | Sunlong Medical

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CBY2; Chibby homolog 2; FLJ35810; Novel leucine zipper testicular protein; NURIT; Protein chibby homolog 2; Spermatid associated; Spermatid associated protein; Spermatid flower like structure protein; Testis specific leucine zipper protein nurit.

Cat:

SL17654R

Species Reactivity：

Human,Rat,(predicted: Mouse,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SPERT:1-100/448

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Hela(human) Cell Lysate at 40 ugDU145(human) Cell Lysate at 40 ugPrimary: Anti-SPERT(SL17654R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52kDObserved band size: 52kDParaformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPERT) Polyclonal Antibody, Unconjugated (SL17654R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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A variety of morphological and molecular changes are required for spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPERT (spermatid associated), also known as CBY2 (chibby homolog 2), spermatid flower-like structure protein or NURIT, is a 448 amino acid novel leucine-zipper protein belonging to the chibby family of proteins.Expressed uniquely in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. Containing a leucine-zipper motif and two coiled-coil regions, SPERT is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies.

Function:
Spermatid-associated protein (SPERT) belongs to the chibby family, SPERT subfamily. It exists as a homodimer and binds to NEK1. There are two named isoforms produced by alternative splicing.

Subcellular Location:
Cytoplasmic membrane bounded vesicle.

Similarity:
Belongs to the chibby family. SPERT subfamily.

SWISS:
Q8NA61

Gene ID:
44082

Database links:

Entrez Gene: 44082 Human

SwissProt: Q8NA61 Human

Unigene: 186363 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPERT) Polyclonal Antibody, Unconjugated (SL17654R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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