Rabbit Anti-SPG11 antibody | Sunlong Medical

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Colorectal carcinoma associated protein; Colorectal carcinoma-associated protein; DKFZp762B1512; FLJ21439; KIAA1840; Spastic paraplegia 11 (autosomal recessive); Spastic paraplegia 11; Spastic paraplegia 11 protein; Spatacsin; SPG 11; Spg11; SP

Cat:

SL17656R

Species Reactivity：

Human,Mouse,(predicted: Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human SPG11:2151-2250/2443

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPG11) Polyclonal Antibody, Unconjugated (SL17656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPG11) Polyclonal Antibody, Unconjugated (SL17656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a potential transmembrane protein that is phosphorylated upon DNA damage. Defects in this gene are a cause of spastic paraplegia type 11 (SPG11). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Subunit:
Interacts with AP5Z1, AP5B1, AP5S1 and ZFYVE26.

Subcellular Location:
Membrane. Cytoplasm > cytosol. Nucleus. Mainly cytoplasmic.

Tissue Specificity:
Expressed in all structures of brain, with a high expression in cerebellum.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE:
Defects in SPG11 are the cause of spastic paraplegia autosomal recessive type 11 (SPG11) [MIM:60472]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.

SWISS:
Q96JI7

Gene ID:
80208

Database links:

Entrez Gene: 80208 Human

Omim: 610844 Human

SwissProt: Q96JI7 Human

Unigene: 656271 Human

Unigene: 683876 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPG11) Polyclonal Antibody, Unconjugated (SL17656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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