Rabbit Anti-ISX/Intestine specific homeobox antibody

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DKFZp781N2395; Intestine-specific homeobox; Isx; ISX_HUMAN; MGC138417; OTTHUMP00000028558; Pancreas intestine homeodomain transcription factor; Pix 1; RAX like homeobox; RAX-like homeobox; RAXLX.

Cat:

SL18172R

Species Reactivity：

Human,Mouse,(predicted: Rat,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox:81-180/245

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Lovo (Human) Cell Lysate at 30 ugLane 2: SW96 (Human) Cell Lysate at 30 ugPrimary: Anti-ISX/Intestine specific homeobox (SL18172R) at 1:500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 27 kDSample: small intestine (Mouse) Lysate at 40 uglarge intestine (Mouse) Lysate at 40 ugPrimary: Anti-ISX/Intestine specific homeobox (SL18172R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 27 kD Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (SL18172R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the RAXLX homeobox gene family. [provided by RefSeq, Jul 2008]

Function:
Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCMO1 expression in the intestine.

Subcellular Location:
Nucleus.

Similarity:
Contains 1 homeobox DNA-binding domain.

SWISS:
Q2M1V0

Gene ID:
91464

Database links:

Entrez Gene: 91464 Human

Omim: 612019 Human

SwissProt: Q2M1V0 Human

Unigene: 567637 Human

Picture

Sample:
small intestine (Mouse) Lysate at 40 ug
large intestine (Mouse) Lysate at 40 ug
Primary: Anti-ISX/Intestine specific homeobox (SL18172R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (SL18172R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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