LNX2 (LIGAND OF NUMB PROTEIN X2, PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 1, PDZRN1), which interacts with mammalian Numb and NumbL, contains a RING finger domain, followed by a motif similar to a PTB-binding motif and 4 PDZ domains. Lnx2 and Lnx1 (a proposed relative) are multimodular proteins that bind Numb, a cell fate determinant, through their NPXY motifs. Studies may suggest that Lnx proteins act as molecular scaffolds that promote the aggregation of unrelated, interacting proteins, such as Numb, to definitive subcellular sites. The LNX2 gene maps to chromosome 13q12.2 based on an alignment of the LNX2 sequence with the genomic sequence. Lnx proteins may form large networks by homomeric binding, and their expression patterns overlap with those of the Numb proteins. Furthermore, studies also suggest that the oligomerization of Lnx2 and Numb binding occurs simultaneously.
Subunit:
Interacts with the phosphotyrosine interaction domain of NUMB.
Similarity:
Contains 4 PDZ (DHR) domains.
Contains 1 RING-type zinc finger.
SWISS:
Q8N448
Gene ID:
222484
Database links:
Entrez Gene: 222484 Human
Omim: 609733 Human
SwissProt: Q8N448 Human
Unigene: 132359 Human
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Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-LNX2 (SL18321R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LNX2) Polyclonal Antibody, Unconjugated (SL18321R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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