Rabbit Anti-LW1/HSFX1 antibody

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hCG_27154; heat shock transcription factor family X linked 1; Heat shock transcription factor, X-linked; HSFX1; HSFX2; LW 1; LW1.

Cat:

SL18448R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human LW1/HSFX1:181-280/423

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Jurkat Cell (Human) Lysate at 40 ugHela Cell (Human) Lysate at 40 ugPrimary: Anti-LW1/HSFX1 (SL18448R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45 kDObserved band size: 48 kD Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (SL18448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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HSFX1 (heat shock transcription factor family, X linked 1), also known as LW-1, is a 423 amino acid protein that is predominately expressed in testis. Localized to the cytoplasm, HSFX1 is thought to be involved in spermatogenesis and male fertility.

Subcellular Location:
Nucleus (Potential). Cytoplasm.

Tissue Specificity:
Testis-specific.

Similarity:
Belongs to the HSF family.

SWISS:
Q9UBD0

Gene ID:
100506164

Database links:

Entrez Gene: 100506164 Human

GenBank: NM_016153 Human

SwissProt: Q9UBD0 Human

Unigene: 709146 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (SL18448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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